Influence of estrogens deficiency on body weight and glucose levels
Keywords:
glucose, blood glucose, body weight, ovariectomy, estrogensAbstract
Introduction: Estrogens deficiency, characteristic of menopause or surgical removal of the ovaries could be related to weight gain and altered glycaemia, but the physiological mechanisms are unknown, generating a scientific gap.
Objective: To assess the influence of estrogens deficiency on body weight and glucose levels.
Methods: Twelve, female, BALB/c mice were equally and randomly divided into two groups: sham surgery and ovariectomy. Mice in the ovariectomy group had their ovaries removed bilaterally, while those in the sham surgery group had their ovaries exposed without removal. Twelve weeks later the mice were sacrificed following the protocol. The weights of the mice were monitored one day before surgery and then monthly for three consecutive months. Preprandial and postprandial glucose levels were also measured before surgery and at 12 weeks after surgery.
Results: An increase in body weight was observed from the first month in favor of the ovariectomized group (p<0.05); also, preprandial and postprandial glucose levels were increased in the ovariectomized group.
Conclusion: Estrogens deficiency leads to increased body weight and altered postprandial and preprandial glucose levels in female mice.
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1. Chandrasekaran P, Weiskirchen R. The Role of Obesity in Type 2 Diabetes Mellitus—An Overview. Int J Mol Sci. 2024;25(3):1882. DOI: 10.3390/ijms25031882.
2. Jeong HG, Park H. Metabolic Disorders in Menopause. Metabolites. 2022;12(10):954. DOI: 10.3390/metabo12100954.
3. Polyzos SA, Anastasilakis AD, Efstathiadou ZA, et al. Postmenopausal osteoporosis coexisting with other metabolic diseases: Treatment considerations. Maturitas. 2021;147:19-25. DOI: 10.1016/j.maturitas.2021.02.007.
4. Purnell JQ, le Roux CW. Hypothalamic control of body fat mass by food intake: The key to understanding why obesity should be treated as a disease. Diabetes Obes Metab. 2024;26(Sup 2):3-12. DOI: 10.1111/dom.15478.
5. Lizcano F, Guzmán G. Estrogen Deficiency and the Origin of Obesity during Menopause. Biomed Res Int. 2014;2014:757461. DOI: 10.1155/2014/757461.
6. Steiner BM, Berry DC. The Regulation of Adipose Tissue Health by Estrogens. Front Endocrinol. 2022;13:889923. DOI: 10.3389/fendo.2022.889923.
7. Obradovic M, Sudar-Milovanovic E, Soskic S, et al. Leptin and Obesity: Role and Clinical Implication. Front Endocrinol. 2021;12:585887. DOI: 10.3389/fendo.2021.585887.
8. Mauvais-Jarvis F, Clegg DJ, Hevener AL. The Role of Estrogens in Control of Energy Balance and Glucose Homeostasis. Endocr Rev. 2013;34(3):309-38. DOI: 10.1210/er.2012-1055.
9. Alemany M. Estrogens and the regulation of glucose metabolism. World J Diabetes. 2021;12(10):1622-54. DOI: 10.4239/wjd.v12.i10.1622.
10. Lei Z, Wu H, Yang Y, et al. Ovariectomy Impaired Hepatic Glucose and Lipid Homeostasis and Altered the Gut Microbiota in Mice With Different Diets. Front Endocrinol. 2021;12:708838. DOI: 10.3389/fendo.2021.708838.
11. Zhang X, Lu JJ, Abudukeyoumu A, et al. Glucose transporters: Important regulators of endometrial cancer therapy sensitivity. Front Oncol. 2022;12:933827. DOI: 10.3389/fonc.2022.933827.
12. Navarro KL, Huss M, Smith JC, et al. Mouse Anesthesia: The Art and Science. ILAR J. 2021;62(1-2):238-73. DOI: 10.1093/ilar/ilab016.
13. Vogel H, Mirhashemi F, Liehl B, et al. Estrogen Deficiency Aggravates Insulin Resistance and Induces β-Cell Loss and Diabetes in Female New Zealand Obese Mice. Horm Metab Res. 2013;45(6):430-5. DOI: 10.1055/s-0032-1331700.
14. Luengo-Mateos M, González-Vila A, Torres Caldas AM, et al. Protocol for ovariectomy and estradiol replacement in mice. STAR Protoc. 2024;5(1):102910. DOI: 10.1016/j.xpro.2024.102910.
15. De la Cruz Rodríguez RA. Influencia de la deficiencia de estrógenos en el hueso alveolar y ligamento periodontal en modelos murinos ovariectomizados [thesis in Internet]. Lima: Universidad Nacional Mayor de San Marcos; 2024 [accessed 2024/12/12]. Available at: https://hdl.handle.net/20.500.12672/24185
16. Souza VR, Mendes E, Casaro M, et al. Description of Ovariectomy Protocol in Mice. Methods Mol Biol. 2019;1916:303-9. DOI: 10.1007/978-1-4939-8994-2_29.
17. Kennard MR, Daniels Gatward LF, Roberts AG, et al. The use of mice in diabetes research: The impact of experimental protocols. Diabet Med. 2021;38(12):e14705. DOI: 10.1111/dme.14705.
18. Al Rijjal D, Wheeler MB. A protocol for studying glucose homeostasis and islet function in mice. STAR Protoc. 2022;3(1):101171. DOI: 10.1016/J.XPRO.2022.101171.
19. Yang HR, Tu TH, Jeong DY, et al. Obesity induced by estrogen deficiency is associated with hypothalamic inflammation. Biochem Biophys Rep. 2020;23:100794. DOI: 10.1016/j.bbrep.2020.100794.
20. Malinská H, Hüttl M, Miklánková D, et al. Ovariectomy-Induced Hepatic Lipid and Cytochrome P450 Dysmetabolism Precedes Serum Dyslipidemia. Int J Mol Sci. 2021;22(9):4527. DOI: 10.3390/ijms22094527.
21. Kuryłowicz A. Estrogens in Adipose Tissue Physiology and Obesity-Related Dysfunction. Biomedicines. 2023;11(3):690. DOI: 10.3390/biomedicines11030690.
22. Gibson CJ, Thurston RC, El Khoudary SR, et al. Body mass index following natural menopause and hysterectomy with and without bilateral oophorectomy. Int J Obes. 2013;37(6):809-13. DOI: 10.1038/ijo.2012.164.
23. Kangudia Mbaya J, Mbanzulu Pita Nsonizau D, Mbanzulu Nsolani N, et al. Blood Glucose Reference Interval in Mus musculus Mice. OALib J. 2023;10(6):1-5. DOI: 10.4236/oalib.1109988.
24. Kim JY, Jo KJ, Kim OS, et al. Parenteral 17beta-estradiol decreases fasting blood glucose levels in non-obese mice with short-term ovariectomy. Life Sci. 2010;87(11-12):358-66. DOI: 10.1016/j.lfs.2010.07.009.
25. Stubbins RE, Najjar K, Holcomb VB, et al. Estrogen alters adipocyte biology and protects female mice from adipocyte inflammation and insulin resistance. Diabetes Obes Metab. 2012;14(1):58-66. DOI: 10.1111/j.1463-1326.2011.01488.x.
26. Aittokallio J, Saaresranta T, Riskumäki M, et al. Effect of menopause and age on vascular impairment. Maturitas. 2023;169:46-52. DOI: 10.1016/j.maturitas.2023.01.006.
27. Bermingham KM, Linenberg I, Hall WL, et al. Menopause is associated with postprandial metabolism, metabolic health and lifestyle: The ZOE predict study. EBioMedicine. 2022;85:104303. DOI: 10.1016/j.ebiom.2022.104303.
28. Yamabe N, Kang KS, Lee W, et al. Estriol blunts postprandial blood glucose rise in male rats through regulating intestinal glucose transporters. Am J Physiol Endocrinol Metab. 2015;308(5):E370-9. DOI: 10.1152/ajpendo.00209.2013.
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Copyright (c) 2025 Ronald Alexis de-la-Cruz-Rodríguez, Lizeth Susana Pacpac-Herrera, César Franco-Quino, Adrián Segundo Mallma-Medina, Eliberto Ruiz-Ramírez, Elías Ernesto Aguirre-Siancas
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