Phytochemical profile, and antimicrobial and antioxidant activities of hydroethanolic extracts of Urera laciniata leaves
Keywords:
antioxidants; antibacterials; Staphylococcus aureus; flavonoids; phenols; Ecuador; medicinal plantsAbstract
Introduction: Ecuador has a rich plant diversity, among which the Urera laciniata, a species with ethnopharmacological potential, stands out.
Objective: To evaluate the antimicrobial and antioxidant activity of hydroethanolic extracts of Urera laciniata leaves dried at different temperatures.
Methods: Total phenols and flavonoids contents were quantified. Antioxidant activity was determined using the DPPH and ABTS assays. Minimum inhibitory and bactericidal concentrations (MIC, MBC) were evaluated by the broth microdilution method. Metabolites were profiled by ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC–MS).
Results: The hydroethanolic extract obtained at 25 ºC was the most active in the microdilution test, inhibiting Staphylococcus aureus ATCC 29213 (MIC 32.25 mg/mL; MBC 62.5 mg/mL). It also presented the lowest IC50 values in antioxidant assays (DPPH 403.82 mg/L; ABTS 894.87 mg/L). Twelve metabolites were identified by UHPLC–MS. The main flavonoids were luteolin-7-glucuronide (match index=0.93) and apigenin-6,8-digalactoside (match index=0.92).
Conclusions: The results support the research and potential applications of U. laciniata, especially in the cosmetic and medicinal fields, due to its antioxidant capacity and its effect against a strain of Staphylococcus aureus associated with skin infections.
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References
1. Mestanza-Ramón C, Henkanaththegedara SM, Vásconez Duchicela P, et al. In-Situ and Ex-Situ Biodiversity Conservation in Ecuador: A Review of Policies, Actions and Challenges. Diversity. 2020;12(8). DOI: 10.3390/d12080315.
2. Elbouzidi A, Haddou M, Baraich A, et al. Biochemical insights into specialized plant metabolites: Advancing cosmeceutical applications for skin benefits. J Agric Food Res. 2025;19:101651. DOI: 10.1016/j.jafr.2025.101651.
3. Radice M, Tasambay A, Pérez A, et al. Ethnopharmacology, phytochemistry and pharmacology of the genus Hedyosmum (Chlorantaceae): A review. J Ethnopharmacol. 2019;244:111932. DOI: 10.1016/j.jep.2019.111932.
4. World Health Organization. WHO global report on traditional and complementary medicine 2019 [Internet]. Geneva: World Health Organization; 2019 [citado 12/01/2026]. Disponible en: https://apps.who.int/iris/bitstream/handle/10665/312342/9789241515436-eng.pdf?ua=1
5. Giovannini P. Medicinal plants of the Achuar (Jivaro) of Amazonian Ecuador: ethnobotanical survey and comparison with other Amazonian pharmacopoeias. J Ethnopharmacol. 2015;164:78-88. DOI: 10.1016/j.jep.2015.01.038.
6. Robles Arias DM, Cevallos D, Gaoue OG, et al. Non-random medicinal plants selection in the Kichwa community of the Ecuadorian Amazon. J. Ethnopharmacol. 2020;246:112220. DOI: 10.1016/j.jep.2019.112220.
7. Stewart VC. Species Delimitation in Neotropical Urera Guadich [Internet]. Edinburgh: The University of Edinburgh; 2018 [citado 12/01/2026]. Disponible en: https://research-scotland.ac.uk/bitstream/20.500.12594/8813/1/2018_Stewart.pdf
8. Mzid M, Ben Khedir S, Ben Salem M, et al. Antioxidant and antimicrobial activities of ethanol and aqueous extracts from Urtica urens. Pharm Biol. 2017;55(1):775-81. DOI: 10.1080/13880209.2016.1275025.
9. Lee U, Eo HJ, Jung CR, et al. Effect of drying temperature on the level of pharmacological compounds in the rhizome of Cnidium officinale Makino. Food Sci Preserv. 2025;32(1):158-64. DOI: 10.11002/fsp.2025.32.1.158.
10. Antony A, Farid M. Effect of Temperatures on Polyphenols during Extraction. Appl Sci. 2022;12(4):2107. DOI: 10.3390/app12042107.
11. Bai AD, Lo CKL, Komorowski AS, et al. Staphylococcus aureus bacteraemia mortality: a systematic review and meta-analysis. Clin Microbiol Infect. 2022;28(8):1076-84. DOI: 10.1016/j.cmi.2022.03.015.
12. Dieguez-Santana K, Casañola-Martin GM, Torres R, et al. Machine learning study of metabolic networks vs ChEMBL data of antibacterial compounds. Mol Pharm. 2022;19(7):2151-63. DOI: 10.1021/acs.molpharmaceut.2c00029.
13. Ezebo R, Okonkwo C, Ozoh C, et al. Phytochemical Screening and Antimicrobial Activity of Ethanol and Methanol Extracts of Lantana camara Leaf. World News Nat Sci [Internet]. 2021 [citado 12/01/2026];37:151-63. Disponible en: https://bibliotekanauki.pl/articles/1839418.pdf
14. Ochoa-Ocampo MA, Niño-Ruiz Z, Torres-Gutiérrez R, et al. Characterization, biological activity and application trends of Ilex guayusa Loes: A systematic literature review and bibliometric analysis. Food Chem Adv. 2025;7:100958. DOI: 10.1016/j.focha.2025.100958.
15. Ochoa-Ocampo M, Espinosa de los Monteros-Silva N, Pastuña-Fasso JV, et al. Volatile Compositional Profile, Antioxidant Properties, and Molecular Docking of Ethanolic Extracts from Philodendron heleniae. Molecules. 2025;30(6):1366. DOI: 10.3390/molecules30061366.
16. Molole GJ, Gure A, Abdissa N. Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin. BMC Chem. 2022;16(1):48. DOI: 10.1186/s13065-022-00841-x.
17. Chandra S, Khan S, Avula B, et al. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study. Evid Based Complement Alternat Med. 2014;2014:253875. DOI: 10.1155/2014/253875.
18. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol. 1995;28(1):25-30. DOI: 10.1016/S0023-6438(95)80008-5.
19. Re R, Pellegrini N, Proteggente A, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231-7. DOI: 10.1016/S0891-5849(98)00315-3.
20. Proaño-Bolaños C, Zhou M, Chen T, et al. Skin secretion transcriptome remains in chromatographic fractions suitable for molecular cloning. Anal Biochem. 2019;564-5:13-5. DOI: 10.1016/j.ab.2018.10.006.
21. Jha AK, Sit N. Extraction of bioactive compounds from plant materials using combination of various novel methods: A review. Trends Food Sci Technol. 2022;119:579-91. DOI: 10.1016/j.tifs.2021.11.019.
22. Shabir S, Yousuf S, Singh SK, et al. Ethnopharmacological Effects of Urtica dioica, Matricaria chamomilla, and Murraya koenigii on Rotenone-Exposed D. melanogaster: An Attenuation of Cellular, Biochemical, and Organismal Markers. Antioxidants. 2022;11(8):1623. DOI: 10.3390/antiox11081623.
23. Mariño Manzano KD. Determinación del efecto antioxidante y antimicrobiano de extractos de diferentes tipos de ortiga (Urtica dioica, Urtica urens, Urtica leptophylla, Urera baccifera) frente a cepas de Staphylococcus aureus, Escherichia coli, Listeria monocytogenes y Bacillus cereus [tesis en Internet]. Ambato: Universidad Técnica de Ambato; 2023 [citado 12/01/2026]. Disponible en: https://repositorio.uta.edu.ec/handle/123456789/37948
24. Borges A, José H, Homem V, et al. Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea. Antibiotics (Basel). 2020;9(2):48. DOI: 10.3390/antibiotics9020048.
25. Modarresi-Chahardehi A, Ibrahim D, Fariza-Sulaiman S, et al. Screening antimicrobial activity of various extracts of Urtica dioica. Rev Biol Trop. 2012;60(4):1567-76. DOI: 10.15517/rbt.v60i4.2074.
26. Velasco Lezama R, Tapia Aguilar R, Ruíz Calvo J, et al. Antibacterial activity of three Urtica species. Int J Pharm Sci Res. 2021;12(2):1293-9. DOI: 10.13040/IJPSR.0975-8232.12(2).1293-99.
27. Tschá MdC, Amorim MJAAL, Farias ETN, et al. Phytochemical profile and evaluation of the antimicrobial activity of the crude hydroalcoholic extract of Cecropia pachystachya leaves. Obs Econ Latinoam. 2024;22(9):e6700. DOI: 10.55905/oelv22n9-091.
28. Crascì L, Basile L, Panico A, et al. Correlating In Vitro Target-Oriented Screening and Docking: Inhibition of Matrix Metalloproteinases Activities by Flavonoids. Planta Med. 2017;83(11):901-11. DOI: 10.1055/s-0043-104775.
29. Wu Z, Lee S, Kang B, et al. Determination of Luteolin 7-Glucuronide in Perilla frutescens (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect. Molecules. 2023;28(20):7007. DOI: 10.3390/molecules28207007.
30. Pérez-Portero Y, Vera-Ayala A, García-Díaz J, et al. Estabilidad físico-química del extracto hidroetanólico al 70% de hojas de Spondias mombin L. Rev Cubana Quím [Internet]. 2021 [citado 12/01/2026];33(3):305-25. Disponible en: https://www.redalyc.org/journal/4435/443569388004/html/
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Copyright (c) 2026 Jairo Livardo Calapucha-Alvarado, Nina Quilla Espinosa-de-los-Monteros-Silva, Melanie Ashley Ochoa-Ocampo, Noroska G. S. Mogollón, Karel Diéguez-Santana
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